An experimental study on production opportunities of biocomposite by using fungal mycelium


  • Sebahat Sevde Sağlam image/svg+xml Istanbul Technical University

    Sebahat Sevde Sağlam is an architect. She is a current master’s student in theDepartment of Environmental Control and Construction Technologies at İstanbul Technical University. Sevde graduated from Maltepe University, Department of Architecture with an honor degree in 2018. After graduation, she worked as an architect for 2 years. Sevde, who is still continuing her graduate studies, conducts research on sustainability and ecological materials. 

  • Seden Acun Özgünler image/svg+xml Istanbul Technical University

    Prof. Dr. Seden Acun Özgünler graduated from Department of Architecture in Yıldız Technical University in 1997.  She completed her M.Arch  and  Ph.D degrees in Building Sciences Programme of İstanbul Technical University. She is a faculty member in the Department of Architecture at Istanbul Technical University. She has studies on Materials and Technology in Architecture. There are many articles, book chapters, and research projects related to her profession. Moreover, she provides consultancy support to private companies and public institutions. 



bio-composite building material, biopolymer, mycelium, mycelium composite, sustainable materials


Due to the adaptability, durability, and affordability of synthetic polymers, their usage has been increasing in the global industry. These petroleum-based polymers remain intact in nature for many years after they expire and cannot be included in the natural recycling network in any way. Producing polymers using fossil resources increasingly day by day threatens existing resources and affects the circular economy negatively. Considering the various negative effects of polymers on the environment, biopolymers could be seen as a strong alternative; which is a polymer group formed by living organisms such as plants, animals, and microorganisms. Ecological, low-emission, and recyclable biopolymers open up new and a broad range of topics in the field. Composite materials created with these biopolymer materials that act as natural adhesives; have different developing areas of applications such as packaging industry, textile, furniture, and industrial design sectors, architectural designs, and structural insulation materials. Fungal mycelium, a biopolymer, consists of fibrous filaments called hyphae, which can be defined as elongated cells, mainly composed of chitin, glucan, and proteins. The ability of fungal mycelium to digest and grow through organic matter makes it possible to produce biocomposites from mycelium. Mycelium-based composites are mixed with fungal mycelium, forming an interpenetrating three-dimensional filamentous network that binds the raw material to the material, and after completing the growth period, the mycelium growth is stopped by heat, thus offering an alternating fabrication paradigm based on the growth of materials.  In this study, firstly, it was tried to find the most efficient ratio among different mixing ratios by using the mycelium of the genus Pleurotus Ostreatus and the same raw materials. Afterward, it was aimed to investigate the mechanical and physical properties through experimental studies, especially the production process, of mycelium-based composites formed by mixing different raw materials in determining proportions.


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  • Abdel-Shafy, H. I., & Mansour, M. S. (2018). Solid waste issue: Sources, composition, disposal, recycling, and valorization. Egyptian journal of petroleum, 27(4), 1275-1290.
  • Ahmadi, H. (2016). Cellulose-Mycelia foam: novel bio-composite material (Doctoral dissertation, University of British Columbia).
  • Alemu, D., Tafesse, M., & Mondal, A. K. (2022). Mycelium-Based Composite: The Future Sustainable Biomaterial. International Journal of Biomaterials, 2022.
  • Appels, F. V., Camere, S., Montalti, M., Karana, E., Jansen, K. M., Dijksterhuis, J., & Wösten, H. A. (2019). Fabrication factors influencing mechanical, moisture-and water-related properties of mycelium-based composites. Materials & Design, 161, 64-71.
  • Attias, N., Danai, O., Ezov, N., Tarazi, E., & Grobman, Y. J. (2017). Developing novel applications of mycelium based bio-composite materials for design and architecture. Proceedings of building with biobased materials: best practice and performance specification, 1(10).
  • Attias, N., Danai, O., Abitbol, T., Tarazi, E., Ezov, N., Pereman, I., & Grobman, Y. J. (2020). Mycelium bio-composites in industrial design and architecture: Comparative review and experimental analysis. Journal of Cleaner Production, 246, 119037.
  • Bitting, S., Derme, T., Lee, J., Van Mele, T., Dillenburger, B., & Block, P. (2022). Challenges and Opportunities in Scaling up Architectural Applications of Mycelium-Based Materials with Digital Fabrication. Biomimetics, 7(2), 44.
  • BS EN 196-1:2016. Methods of testing cement. Determination of strength.
  • BS EN 1097-6:2022. Tests for mechanical and physical properties of aggregates. Determination of particle density and water absorption.
  • de Caprariis, B., De Filippis, P., Petrullo, A., & Scarsella, M. (2017). Hydrothermal liquefaction of biomass: Influence of temperature and biomass composition on the bio-oil production. Fuel, 208, 618-625.
  • Dias, P. P., Jayasinghe, L. B., & Waldmann, D. (2021). Investigation of Mycelium-Miscanthus composites as building insulation material. Results in Materials, 10, 100189.
  • Elbasdı, A. G. (2016). An Investigation on Growth Behavior of Mycelium
  • -Based Material In A Fabric Formwork (Doctoral dissertation, Fen Bilimleri Enstitüsü).
  • Elsacker, E., Vandelook, S., Brancart, J., Peeters, E., & De Laet, L. (2019). Mechanical, physical and chemical characterisation of mycelium-based composites with different types of lignocellulosic substrates. PLoS One, 14(7), e0213954.
  • Elsacker, E., Vandelook, S., Van Wylick, A., Ruytinx, J., De Laet, L., & Peeters, E. (2020). A comprehensive framework for the production of mycelium-based lignocellulosic composites. Science of The Total Environment, 725, 138431.
  • Etinosa, O. P. (2019). Design and Testing of Mycelium Biocomposite. (Doctoral dissertation).
  • Elsacker, E., Søndergaard, A., Van Wylick, A., Peeters, E., & De Laet, L. (2021). Growing living and multifunctional mycelium composites for large-scale formwork applications using robotic abrasive wire-cutting. Construction and Building Materials, 283, 122732.
  • Faruk, O., Bledzki, A. K., Fink, H. P., & Sain, M. (2012). Biocomposites reinforced with natural fibers: 2000–2010. Progress in polymer science, 37(11), 1552-1596.
  • Gauvin, F., Tsao, V., Vette, J., & Brouwers, H. J. H. (2022). Physical Properties and Hygrothermal Behavior of Mycelium-Based Composites as Foam-Like Wall Insulation Material. In Construction Technologies and Architecture (Vol. 1, pp. 643-651). Trans Tech Publications Ltd.
  • Girometta, C., Picco, A. M., Baiguera, R. M., Dondi, D., Babbini, S., Cartabia, M., ... & Savino, E. (2019). Physico-mechanical and thermodynamic properties of mycelium-based biocomposites: a review. Sustainability, 11(1), 281.
  • Haneef, M., Ceseracciu, L., Canale, C., Bayer, I. S., Heredia-Guerrero, J. A., & Athanassiou, A. (2017). Advanced materials from fungal mycelium: fabrication and tuning of physical properties. Scientific reports, 7(1), 1-11.
  • Heisel, F., Lee, J., Schlesier, K., Rippmann, M., Saeidi, N., Javadian, A., ... & Hebel, D. E. (2017). Design, Cultivation and Application of Load-Bearing Mycelium Components: The MycoTree at the 2017 Seoul Biennale of Architecture and Urbanism. International Journal of Sustainable Energy, 6(1), 18.
  • Heisel, F., & Rau-Oberhuber, S. (2020). Calculation and evaluation of circularity indicators for the built environment using the case studies of UMAR and Madaster. Journal of Cleaner Production, 243, 118482.
  • ISO 9427:2003. Wood-based panels—Determination of density.
  • ISO 16979:2003. Wood-based panels—Determination of moisture content.
  • Jahirul, M. I., Rasul, M. G., Chowdhury, A. A., & Ashwath, N. (2012). Biofuels production through biomass pyrolysis—a technological review. Energies, 5(12), 4952-5001.
  • Javadian, A., Le Ferrand, H., Hebel, D. E., & Saeidi, N. (2020). Application of mycelium-bound composite materials in construction industry: a short review. SOJ Materials Science and Engineering, 7, 1-9.
  • Jiang, L., Walczyk, D., McIntyre, G., Bucinell, R., & Tudryn, G. (2017). Manufacturing of biocomposite sandwich structures using mycelium-bound cores and preforms. Journal of Manufacturing Processes, 28, 50-59.
  • Jones, M., Bhat, T., Huynh, T., Kandare, E., Yuen, R., Wang, C. H., & John, S. (2018). Waste‐derived low‐cost mycelium composite construction materials with improved fire safety. Fire and Materials, 42(7), 816-825.
  • Jones, M., Mautner, A., Luenco, S., Bismarck, A., & John, S. (2020). Engineered mycelium composite construction materials from fungal biorefineries: A critical review. Materials & Design, 187, 108397.
  • Joshi, K., Meher, M. K., & Poluri, K. M. (2020). Fabrication and characterization of bioblocks from agricultural waste using fungal mycelium for renewable and sustainable applications. ACS Applied Biomaterials, 3(4), 1884-1892.
  • Kuribayashi, T., Lankinen, P., Hietala, S., & Mikkonen, K. S. (2022). Dense and continuous networks of aerial hyphae improve flexibility and shape retention of mycelium composite in the wet state. Composites Part A: Applied Science and Manufacturing, 152, 106688.
  • Lee, T., & Choi, J. (2021). Mycelium-composite panels for atmospheric particulate matter adsorption. Results in Materials, 11, 100208.
  • Lelivelt, R. J. (2015). The production process and compressive strength of mycelium-based materials. In First International Conference on Bio-based Building Materials. (s. (pp. 1-6).). France: Clermont-Ferrand.
  • Moser, F., Trautz, M., Beger, A. L., Löwer, M., Jacobs, G., Hillringhaus, F., ... & Reimer, J. (2017, September). Fungal mycelium as a building material. In Proceedings of IASS Annual Symposia (Vol. 2017, No. 1, pp. 1-7). International Association for Shell and Spatial Structures (IASS).
  • Rafiee, K., Kaur, G., & Brar, S. K. (2021). Fungal biocomposites: How process engineering affects composition and properties? Bioresource Technology Reports, 14, 100692.
  • Silverman, J. (2018). Development and testing of mycelium-based composite materials for shoe sole applications. University of Delaware.
  • Sivaprasad, S., Byju, S. K., Prajith, C., Shaju, J., & Rejeesh, C. R. (2021). Development of a novel mycelium bio-composite material to substitute for polystyrene in packaging applications. Materials Today: Proceedings, 47, 5038-5044.
  • Travaglini, S., Dharan, C. K. H., & Ross, P. (2016). Manufacturing of mycology composites. In Proceedings of the American Society for Composites: Thirty-First Technical Conference.
  • Udayanga, D., & Miriyagalla, S. D. (2021). Fungal Mycelium-Based Biocomposites: An Emerging Source of Renewable Materials. In Microbial Technology for Sustainable Environment (pp. 529-550). Springer, Singapore.
  • Vasquez, E. S. L., & Vega, K. (2019, September). From plastic to biomaterials: prototyping DIY electronics with mycelium. In Adjunct Proceedings of the 2019 ACM International Joint Conference on Pervasive and Ubiquitous Computing and Proceedings of the 2019 ACM International Symposium on Wearable Computers (pp. 308-311).
  • Yang, Z., Zhang, F., Still, B., White, M., & Amstislavski, P. (2017). Physical and mechanical properties of fungal mycelium-based biofoam. Journal of Materials in Civil Engineering, 29(7), 04017030.
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How to Cite

Sağlam, S. S., & Acun Özgünler, S. (2022). An experimental study on production opportunities of biocomposite by using fungal mycelium. Journal of Design for Resilience in Architecture and Planning, 3(2), 237–260.



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